Efficiently Build Relevant In Vitro Models Using Human Stem Cell-Derived Tissue Cells, High Performance Transfection and Novel Multiplexed Reporter Techniques (Efficiently Build Relevant In Vitro Models Using Human Stem Cell-Derived Tissue Cells, High Performance Transfection and Novel Multiplexed Reporter Techniques)
Owner/Developer: Promega
| Country: |
United States of America |
|---|---|
| Languages: |
English |
| Url: |
https://se.promega.com/resources/webinars/worldwide/archive/buildyourrelevant_invitro_models_easily/ |
| Created: |
09 December 2014 |
| Description: |
This webinar will discuss how highly pure human tissue cells and new transfection techniques can be used to build relevant human cell models that recapitulate disease phenotypes and enable rapid, quantitative analysis of biological pathways. |
| References: |
ppt slides:
https://promega.media/-/media/files/promega-worldwide/north-america/promega-us/webinars-and-events/2014/promega-cdi-build-relevant-invitro-models-dec2014.pdf?la=en |
| Format: |
Webinars |
| Presence: |
Optional / Voluntary |
| Access: |
Free |
| Content type: |
Theoretical |
| Target audience: |
Students, Researchers, Regulators and policy-makers, Teachers and educators, Technicians, Managers, Scientific officers / Project managers, Professionals (e.g. veterinarians), General public |
| Target sectors: |
Academia, Industry, Governmental bodies, Contract Research Organizations (CROs), Consulting, SMEs |
| Educational level: |
University (Bachelor), University (Master), University (Doctoral education), Postdoctoral (teaching and research), Continuing Professional Development |
| 3rs relevance: |
Replacement |
| Topics covered: |
In vitro methods |
| 3rs coverage: |
Full coverage (a dedicated course) |
| Details on the topic or technology covered: |
Scientific and technical barriers to understanding human biology are being removed. The commercial availability of highly pure human tissue cells, such as iCell® Cardiomyocytes and iCell DopaNeurons, has introduced physiologically relevant cellular models into basic research, early drug discovery and development, and late-stage pre-clinical studies. Genetic manipulation of these cell models using ViaFect™ Transfection Reagent, an advanced lipid-based transfection technique enabling highly efficient and low toxicity transfection of reporter constructs and markers, offers the opportunity to build relevant human cell models that recapitulate disease phenotypes and enable rapid and quantitative analysis of biological pathways.
Scientists from Cellular Dynamics International and Promega co-presented this webinar describing the application and advantages of genetically manipulating human iCell products with ViaFect. You will walk away with: New insight into highly efficient and low toxicity transfection of human cells Examples of multiplexing cellular changes with functional readouts New techniques for easy implementation of relevant human biology into workflows |
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