ELISA Advanced Course
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In this ELISA course, you will be introduced to the possibilities of ELISA techniques in theory and practice, and you will develop the competence needed to establish these techniques in your laboratory. The theoretical module covers the possibilities and parameters of different methods while in the practical part, you will use this theory to solve an analytical problem (determination of the degree of contamination in a product using a biochemical assay). In order to do so, you will independently establish an ELISA system and optimize it with assistance. Thereafter, a systematical error analysis is discussed and possible short-comings of the system will be identified and removed.
Hands-on training, Lecture
Optional / Voluntary
Technical and scientific staff members, who want to establish or optimize new ELISA techniques or who want to develop an ELISA.
Students, Researchers, Teachers and educators, Technicians, Managers, Scientific officers / Project managers
Academia, Industry, Contract Research Organizations (CROs), Consulting, SMEs
University (Bachelor), University (Master), University (Doctoral education), Postdoctoral (teaching and research), Continuing Professional Development
In vitro methods
Full coverage (a dedicated course)
|Details on the topic or technology covered:||
The course covers:
Short introduction to ELISA techniques and important vocabulary
Components of ELISA systems
Generation of antibody conjugates
Possibilities for optimization of components
Choice of optimal ELISA concepts
Optimization of robustness and stability of the test system
Development strategy – from assessment of demand to the final assay
Determination of capacity – verification and validation of an ELISA
Trouble shooting by systematic error analysis
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