The vital-microscopy technique and some examples of its use to show dynamic processes in living tissue.
Tor Brustad
Department of Biophysics, Institute of Cancer Research and
Department of Medical Physics and Technology, The Norwegian Radium Hospital, Oslo, Norway
A technique is described by which a very thin layer of living tissue becomes localized in the space between two optical windows in a so-called transparent chamber, implanted in a skin flap on the back of normal or athymic mice.
One of the chamber windows can - whenever desired - be removed temporarily, for precise deposition by micromanipulation technique of - for instance - aliquots of a given drug or a 'tumor seed' onto a predetermined location in the tissue, prior to closing the chamber with a new window. Alternatively the chamber tissue or a given part of it can be irradiated.
Processes in the chamber tissue initiated by such treatments, can be followed by video recording through an appropriate microscopic setup, in normal or time lapse mode.
A few examples will be presented to exemplify the numerous applications of this powerful technique to visualize and to study dynamic processes in viable normal, irradiated and neoplastic chamber tissues.
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