Advanced Protocols for Animal Transgenesis

This laboratory manual provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. Related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R's refinement, reduction and replacement. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their own experience the autors also provide helpful notes and troubleshooting sections. 

661 pages. Available as a Hardcover, eBook and Enhanced eBook. This book may be purchased here. Published in cooperation with the International Society for Transgenic Technology (ISTT). Part of the Springer Protocols Handbooks. Table of Contents.

ISBN: Softcover: 978-3-662-51943-1, Hardcover: 978-3-642-20791-4; eBook: 978-3-642-20792-1

Softcover: €174.99, Hardcover: €199.99, eBook: €139.09