3D Cell Cultures and Tissue Clearing: A High Content Confocal Platform for the Complete 3D Characterization of Advanced Cell Models
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Owner/Developer: Labroots
| Country: |
United States of America |
|---|---|
| Languages: |
English |
| Url: |
https://www.labroots.com/webinar/3d-cell-cultures-tissue-clearing-content-confocal-platform-complete-3d-characterization-advanced-cel |
| Created: |
09 August 2018 |
| Founding source: |
Thermo Fisher Scientific |
| Format: |
Webinars |
| Presence: |
Optional / Voluntary |
| Access: |
Fee-based |
| Content type: |
Theoretical |
| Duration: |
1 h |
| Target audience: |
Students, Researchers, Regulators and policy-makers, Teachers and educators, Managers, Scientific officers / Project managers |
| Target sectors: |
Academia, Industry, Governmental bodies, Contract Research Organizations (CROs), Consulting, SMEs |
| Educational level: |
University (Bachelor), University (Master), University (Doctoral education), Postdoctoral (teaching and research), Continuing Professional Development |
| 3rs relevance: |
Replacement |
| Topics covered: |
In vitro methods |
| 3rs coverage: |
Full coverage (a dedicated course) |
| Details on the topic or technology covered: |
With the advent of cost-effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-environment; showing improved predictive utility for assessing drug efficacy and/or toxicity when compared to traditional 2D monolayer models. High-content analysis/screening (HCA/HCS) also plays a major role in drug screening, but one of the unique challenges of evaluating 3D-CCMs (i.e. spheroids, organoids, micro-tissues, organs-on-a-chip) is their opacity and thickness that limit optical imaging to only the outermost layers (~20=30 microns). Since the outermost cells are exposed to different physiological test conditions (oxygen, nutrients, media exchange, drug dosing), current image-based approaches induce a bias in the results since the outer layer of cells are found in a significantly different micro-environment than the interior. This shortcoming is particularly problematic when ascertaining the relative effectiveness of current therapeutic agents (e.g. immunoglobulin-based therapeutics, anti-proliferatives) since their effects are likely to be localized or concentrated to the surface while their efficacy within the interior are obfuscated and not fully resolved. Visikol™ has shown that through the addition of high content confocal microscopy with the Thermo Scientific™ CellInsight™ CX7 LZR High-Content Analysis Platform and the Invitrogen™ CytoVista™ 3D Cell Culture Clearing Reagent that the entire population of cells within 3D-CCMs can be characterized. |
| Learning outcome: |
Learning Objectives: Benefits of imaging 3D Cell Cultures Practical considerations for buying a High-Content Analysis Platform |
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