Cell Health and Viability
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Owner/Developer: American Type Culture Collection (ATCC)
| Country: |
United States of America |
|---|---|
| Languages: |
English |
| Url: |
https://www.lgcstandards-atcc.org/About/News_and_Events/Events/Webinars/2017/Cell_Health_and_Viability.aspx |
| Created: |
14 September 2017 |
| Description: | Assessing cell viability and cell health is crucial to all aspects of cell culture. In addition to the proper maintenance of cell stocks, cell health practices increase the level of confidence in experimental results. In this webinar presentation, we will discuss reliable and economic methods for determining cell proliferation and cell viability. Other cell culture practices and issues that affect cell health will also be addressed. These topics include choices in cell culture media/reagents, cryopreservation factors that affect cell lines and primary cells, and hard-to-detect mycoplasma contamination. |
| Format: |
Webinars |
| Presence: |
Optional / Voluntary |
| Access: |
Free |
| Content type: |
Theoretical |
| Duration: |
33 min |
| Target audience: |
Students, Researchers, Regulators and policy-makers, Teachers and educators, Technicians, Managers, Scientific officers / Project managers, Professionals (e.g. veterinarians), General public |
| Target sectors: |
Academia, Industry, Governmental bodies, Contract Research Organizations (CROs), Consulting, SMEs |
| Educational level: |
University (Bachelor), University (Master), University (Doctoral education), Postdoctoral (teaching and research), Continuing Professional Development |
| 3rs relevance: |
Replacement |
| Topics covered: |
In vitro methods |
| 3rs coverage: |
Full coverage (a dedicated course) |
| Details on the topic or technology covered: |
Key Points: Cell viability/proliferation can be measured quickly and easily with a number of assays, including the ATCC MTT and XTT proliferation kits, as well as the new Reliablue™ cell viability regent. When culturing specialty cells, such as stem cells or primary cells, certain considerations with choice of media and reagents must be taken. Mycoplasma contamination can cause chronic physiological problems with cultured cells and are hard to detect visually, but can be easily assessed with Mycoplasma Detection Kits and/or services, provided by ATCC. |
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