Hands-on Basic Cell Culture
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Owner/Developer: LabMagister Training and Science Ltd.
| Country: |
Hungary |
|---|---|
| Languages: |
English |
| Url: |
https://www.labmagister.com/may/basic%20cell%20culture |
| Created: |
13 March 2013 |
| Locations: |
Hungary |
| City: |
Budapest |
| Description: | Basic cell culture hands-on course offers a good opportunity to be familiar with and get practice of doing steril work with a cell line in a biosafety cabinet. The different tools like pipettes, pipettors, stirrers, centrifuges, microscopes, hemocytometer and the use of them will be introduced to the trainees. They will get a practice of tawing frozened cells from liquid nitrogen, preparation of the cell for culturing in a cell culture medium. Cell counting with hemocytometer and doing viability test with BD ViCell equipment is also a part of the program. The trainees will have the opportunity to make cell passage and froze down cell aliquots in liquid nitrogen by their own hands. The course is 3-day long. If a trainee is going to spend more days with practicing cell culturing LabMagister’s staff is ready to serve this need for an extra fee. |
| Format: |
Hands-on training |
| Access: |
Fee-based |
| Content type: |
Practical |
| Duration: |
3 days |
| Target audience: |
Students, Researchers, Technicians |
| Target sectors: |
Academia, Industry, Contract Research Organizations (CROs) |
| Educational level: |
Continuing Professional Development |
| 3rs relevance: |
Replacement |
| Topics covered: |
In vitro methods |
| 3rs coverage: |
Partial coverage (e.g. a module) |
| Course level on animal species: |
Basic course |
| Details on the topic or technology covered: |
Rules of steril work; self protection; biosafety cabinet; use of pipettes and pipettors; rules of dilutions; cell frozing; cell passage; introduction of hemocytometer; cell dilution; use of laboratory centrifuge;viability test; keeping clean the workstation;safety instructions. |
| Learning outcome: |
The trainees will be familiar with doing steril cell culture work in a biosafety cabinet. This knowlegde and practice can be applicable for any other cell culture work. The introduction of different pipettes, pipettors, microscopes, hemocytometer, handling disposals, keeping clean the workstation helps the trainees in doing other lab works than cell culturing too. |
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