In vitro research method for screening inhibitors of protein translation
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Owner/Developer: Labroots
| Country: |
United States of America |
|---|---|
| Languages: |
English |
| Url: |
https://www.labroots.com/webinar/vitro-research-method-screening-inhibitors-protein-translation-2 |
| Created: |
17 May 2018 |
| Format: |
Webinars |
| Presence: |
Optional / Voluntary |
| Access: |
Free |
| Content type: |
Theoretical |
| Target audience: |
Students, Researchers, Regulators and policy-makers, Teachers and educators, Scientific officers / Project managers, Professionals (e.g. veterinarians) |
| Target sectors: |
Academia, Industry, Governmental bodies, Contract Research Organizations (CROs), Consulting, SMEs |
| Educational level: |
University (Bachelor), University (Master), University (Doctoral education), Postdoctoral (teaching and research), Continuing Professional Development |
| 3rs relevance: |
Replacement |
| Topics covered: |
In vitro methods |
| 3rs coverage: |
Full coverage (a dedicated course) |
| Details on the topic or technology covered: |
In vitro translation (IVT), or cell-free expression, offers a unique and powerful research tool to screen for translational inhibitors that regulate both cellular and viral protein expression. Here we discuss a simple, quick method to identify inhibitors of both cap-dependent and cap-independent protein translation, using the Thermo Scientific1-Step Human Coupled IVT Kit. Rapid assay readout (6090 min), amenability for miniaturization, and insensitivity to compound toxicity make in vitro translation an attractive alternative to cell-based screens for high-throughput screening (HTS) of novel inhibitors of proteinsynthesis. |
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