Molecular Biology Basic Course
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a dataset collected by the EU Commission in June-September 2018
. Some of these links will therefore die out with time.
Please see the
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Owner/Developer: PromoCell
| Country: |
Germany |
|---|---|
| Languages: |
English |
| Url: |
https://www.promocell-academy.com/programm/courses-in-english/molecular-biology-basic-course/ |
| Locations: |
Germany |
| City: |
Heidelberg |
| Description: | Molecular biology focuses on the structure, biosynthesis and function of DNA and RNA at the molecular level and how these interact with each other and with proteins. Molecular biology techniques including PCR, cloning, mutagenesis, and recombinant expression, are essential for modern biological and medical research. This course will give you an introduction to standard DNA and RNA techniques |
| Format: |
Hands-on training, Lecture |
| Presence: |
Optional / Voluntary |
| Access: |
Fee-based |
| Content type: |
Theoretical, Practical |
| Duration: |
4 days |
| Group size: |
8 |
| Frequency: |
Recurrent event |
| Prerequisites: |
Technical and scientific staff members without any previous knowledge or those possessing only a basic knowledge of molecular biology. |
| Target audience: |
Students, Researchers, Regulators and policy-makers, Teachers and educators, Technicians, Managers, Scientific officers / Project managers, Professionals (e.g. veterinarians) |
| Target sectors: |
Academia, Industry, Governmental bodies, Contract Research Organizations (CROs), Consulting, SMEs |
| Educational level: |
University (Bachelor), University (Master), University (Doctoral education), Postdoctoral (teaching and research), Continuing Professional Development |
| 3rs relevance: |
Replacement |
| Topics covered: |
In vitro methods |
| 3rs coverage: |
Full coverage (a dedicated course) |
| Details on the topic or technology covered: |
The theoretical part includes: Basic knowledge of molecular biology Structure and synthesis of macromolecules Principles of cloning Cloning vectors Restriction enzymes Polymerase Chain Reaction (PCR) Gene regulation and gene expression The practical part includes: Plasmid preparation Determination of nucleic acid concentrations RNA isolation and reverse transcription Polymerase Chain Reaction (PCR) DNA gel electrophoresis, documentation, gel extraction Restriction digest, vector dephosphorylation, ligation of DNA Generation of competent cells and their transformation Over-night cultures and selection on plates, blue white selection Restriction analysis |
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