Transgenic Mouse Methods and Protocols

This book provides descriptions of how to generate conventional knockout and trangenic mice, and offers cutting-edge methods for generation of transgenic mouse models. The book also incudes detailed protocols for vector preparation of different kinds of mouse models. The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In this book, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the "Methods in Molecular Biology" series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Second Edition. XI + 352 pages. Hardcover, Softcover & eBook. A Product of Humana Press. Part of the Methods in Molecular Biology Series. Please see record numbers 8418 and 8431 for two other books in the same Series. Each chapter may be downoladed as PDFs immediately after purchasing. Table of Contents.

ISBN: Hardcover: 978-1-60761-973-4; Softcover: 978-1-4939-5676-0, e-Book: 978-1-60761-974-1

Price: Hardcover: EUR219.99; Softcover: EUR154.99, e-Book: EUR128.39