Performance of the zebrafish embryo test for acute toxicity
This record is part of
a dataset collected by the EU Commission in June-September 2018
. Some of these links will therefore die out with time.
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Owner/Developer: VITO (VITO)
01 December 2015
Hands on training including steps maintenance zebrafish, set up breeding pairs, collection and evaluation of fertilized zebrafish eggs, experimental set up and exposure to test solutions, positive and negative control, evaluation procedure & data analysis (see OECD guideline 236, or ISO 15088 and Flemish WAC/V/B/002 procedure)
Documents on line available : OECD guideline 236 & Flemish Guideline WAC/V/B/002 through EMIS website
Course documentation & materials available at VITO
Students, Researchers, Technicians
Academia, Industry, Contract Research Organizations (CROs)
High school, Technical College, Undergraduate, University (Bachelor), University (Master), University (Doctoral education), Postdoctoral (teaching and research), Continuing Professional Development
Carrying out procedures on animals, Taking care of animals, In vitro methods
Full coverage (a dedicated course)
Zebrafish (Danio rerio)
|Details on the topic or technology covered:||
Replacement of acute toxicity test with juvenile fish, by zebrafish embryo (< 5 days) considered a non-animal model during 1st days of development according to EU legislation. This is an alternative model, but whole organism approach
Directive 2010/63/EU or equivalent, REACH Regulation (EC) No 1907/2006 or equivalent, Test guidelines (OECD, ISO, etc.)
practical training & theoretical background to perform the zebrafish embryo test
Certificate of training is given (VITO document)
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