3D DYNAMIC CELL CULTURE AND IN-VITRO ALTERNATIVE SYSTEMS (3D DYNAMIC CELL CULTURE AND IN-VITRO ALTERNATIVE SYSTEMS)
United States of America
14 October 2015
United States of America
Current in-vitro models are poorly representative of human pathophysiology. Although the recent efforts to fill the gap between in-vitro and in-vivo reality (i.e. 3D in-vitro models, 3D organ printing) and the increase of attention given to the 3Rs principles, the in-vitro models are still brutal approximations of the physiological human environment. Thus their use in the pre-screening testing (i.e. toxicity tests) of new substances is still related to niche applications. This is mostly due to several limitations of the standard equipment used in biological laboratories, e.g. petri dishes and multiwell plates, that lack of the dynamic conditions of the human environment and the cross talk between different tissues. Recently more attention has been focused on the 3D architecture of in-vitro tissues, in particular using innovative techniques such as the 3D bioprinting. Although the progress in the topological correlation between in-vitro and in-vivo tissues, recreating pathophysiologically relevant environments in-vitro is still challenging. This drawback led to a large use of animal models in drug testing, despite their acknowledged lack of human predictivity. Thus the reduction of animal experiments can only be brought about by adopting new cell culture technology which better recapitulates the dynamic physiological environment and its stimuli.
IVTech is dedicated to the dissemination of new methods and approaches to cell culture experiments which bring in-vitro systems closer to the in-vivo environment, by combining flow, scaffolds and multiple cell types in fluidic bioreactors.
Optional / Voluntary
Students, Researchers, Regulators and policy-makers, Teachers and educators, Technicians, Managers, Scientific officers / Project managers, Professionals (e.g. veterinarians), General public
Academia, Industry, Governmental bodies, Contract Research Organizations (CROs), Consulting, SMEs
In vitro methods
Full coverage (a dedicated course)
Cosmetics Regulation (EC) No 1223/2009 or equivalent
In this workshop you will learn about 3D cell culture models that closely represent the human physiology. In particular the subject of the workshop will be:
Introduce the theory of cell culture in 3D under flow conditions, with reference to models of shear stress and oxygen consumption
Demonstrate innovative cell culture systems to develop an in-vitro model
Show participants how to perform an experiment using 3D scaffolds in dynamic conditions
Demonstrate how to determine endpoints and how to analyse the results from a 3D in-vitro model
Provide the participants with hands-on experience in setting up a simple 3D dynamic in-vitro model
You will gain the necessary knowledge implement a 3D dynamic in-vitro model, from theory to practice.
Did you find what you were looking for?Yes, I found it! No, I did not!
Thanks for your feedback! Please note that we cannot respond unless you supply your email address.
What are you looking for?
Please give us your feedback so we can improve the information on the page. Thank you in advance for your help. Please add your email address if you would like a reply.Please contact us by email if you have any questions.