Identification method and tissue sampling for genotyping
|
Source |
Non-harmful / below threshold / severity degree 0 | Mild / severity degree 1 | Moderate / severity degree 2 | Severe / severity degree 3 |
| Directive 2010/63/EU, Annex VIII |
Superficial procedures, e.g. ear and tail biopsies. | |||
| Home Office (2014 b) | Invasive genotyping carried out using a method that causes only transient pain, even if the phenotype is not harmful. | |||
| Federal Food Safety and Veterinary Office FSVO (2018) | Experimental marking and genotyping using non-invasive methods. Examples: Marking with dye. Genotyping by hair sample. |
Experimental marking using invasive methods and tissue collection under anaesthesia for the genotyping of adult animals. Examples: Tattooing. Ear punching, Microchips in rodents and rabbits. Experimental tail tip biopsy up to 0.5 cm. Toe tip amputation up to the age of 3 weeks. |
Experimental interventions to regulate reproduction or for genotyping of adult animals. Examples: Vasectomy. Castration. |
Germ cells
|
Source |
Non-harmful / below threshold / severity degree 0 | Mild / severity degree 1 | Moderate / severity degree 2 | Severe / severity degree 3 |
| Federal Food Safety and Veterinary Office FSVO (2018) |
Collection of germ cells or embryos for experimental purposes from dead parent animals, including females hormonally pretreated to induce superovulation Use of eggs from fish and amphibians if the developing larvae are killed before hatching. Examples: Retrieval of eggs from mice. Collection of embryos from rats. | Use of embryos, foetuses or larvae for experimental purposes if they survive the date of birth, hatching or metamorphosis and only mild impairments of the animals are expected. |
Use of embryos, foetuses or larvae for experimental purposes if they survive the date of birth, hatching or metamorphosis and only moderate impairments of the animals are expected. Collection of germ cells or non-viable developmental stages for experimental purposes from living parent animals (all species including Xenopus). Examples: Mouse strains produced using oncogenes, if the termination criteria are selected accordingly. Chemical and radiation-induced mutagenesis for the production of animal lines with defects. |
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