Novel Zebrafish (Danio rerio) Assay for Assessing Musk Ambrette-Induced Toxicity
By Li Qu - Chaomin Zhao - Chuanxian Wang - Shuqing Gu - Minchao Zhang - Xiaojun Deng - Weimin Song
Record number: | 6010350 |
---|---|
Category: | Danio rerio - acute toxicity - angiogenesis - caudal vein - death - edema - embryo (animal) - genetically modified organisms - humans - lethal concentration 50 - models - pericardium - permeability - pollutants - soaking - animal use alternatives |
Musk ambrette (4-tert-butyl-3-methoxy-2, 6-dinitrotoluene) is a nitro musk, a cheap substitute for natural musk and a potential environmental pollutant based on its persistence, accumulation in human organisms. We investigated the acute toxicity of musk ambrette using wild-type AB and transgenic Tg(fli1a:EGFP)ʸ¹ zebrafish. Different concentrations were delivered to zebrafish by direct soaking from 6 to 72 h post-fertilization (hpf). The LC₅₀ of musk ambrette was 76.4 µg mL⁻¹. As musk ambrette concentration increased, zebrafish embryos showed developmental delays (50 µg mL⁻¹, 22 hpf), pericardial edema (5 µg mL⁻¹, 48 hpf), circulatory disturbances, curved body axis (1 µg mL⁻¹, 72 hpf) and death (100 µg mL⁻¹, 22 hpf). Target organ toxicity was evaluated by a zebrafish angiogenesis model. Musk ambrette induced cardiovascular morphological changes, vessel permeability variation, angiogenic changes and cardiotoxicity (10 µg mL⁻¹, 48 hpf). The disappearance of caudal vein plexus confirmed the vascular development toxicity. Musk ambrette negatively affects early life-stage survival and demonstrates various toxicities in zebrafish.
Issued: 2018
Journal Title: Bulletin of environmental contamination and toxicology
ISSN: 0007-4861
Volume issue: 101
Pages: 80 - 85
Publisher: Springer US
AGRICOLA identifier: IND606010350
DOI identifier: 10.1007/s00128-018-2363-z
Did you find what you were looking for?
Yes, I found it! No, I did not!Thanks for your feedback! Please note that we cannot reply to you unless you send us an email.
What are you looking for?
We value your feedback so we can improve the information on the page. Please add your email address if you would like a reply. Thank you in advance for your help.!
Please contact us by email if you have any questions.