Saphenous vein puncture for blood sampling of the mouse, rat, hamster, gerbil, guineapig, ferret and mink
| Record number: | a833b (legacy id: 15080) |
|---|---|
| Category: | Blood sampling |
| Type: | Guidelines |
| Relevance: | Refinement |
The paper describes a method of blood sampling from the lateral saphenous vein. This enables rapid sampling, which if necessary can be repeated from the same site without a need for new puncture wounds. The method is a humane and practical alternative to cardiac and retro-orbital puncture, in species where venepuncture has traditionally been regarded as problematic.
Hem A, Smith AJ & Solberg P (1998): Saphenous vein puncture for blood sampling of the mouse, rat, hamster, gerbil, guineapig, ferret and mink. Laboratory Animals, 32: 364-368.
The paper can be downloaded as a pdf-file.
See this link for pictures and film of the technique. Please note that this is an old film, and there may be more refined methods for immobilising the animal.
Links to more guidance on bleeding animals are provided here in the PREPARE guidelines.
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