Functionally Characterized Human PBMCs An Improved In Vitro Model of Human Immune Response (Functionally Characterized Human PBMCs An Improved In Vitro Model of Human Immune Response)
Owner/Developer: American Type Culture Collection (ATCC)
United States of America
30 June 2016
Human Peripheral Blood Mononuclear Cells (PBMCs) contain many of the functional cell types of immune system, and are an ideal model to study the human immune response in vitro. Currently, PBMCs are being used in basic and clinical research areas including basic immunology and cell biology, infectious diseases, vaccine development, tumor immunology, and drug discovery. PBMC are also widely used for monitoring disease progression, designing personalized approaches to treatment, and predicting the occurrence of treatment-associated adverse events. The majority of PBMC applications rely on highly complex cell-based assays; however, phenotypic and functional variability of human PBMCs makes the development and validation of these assays difficult. Additionally, variations in cell collection and cryopreservation protocols can further affect PBMC properties. In this presentation, we demonstrate that pre-screening characterization of lot-specific functional activity of PBMCs is extremely useful for selecting cells to address individual experimental goals
Optional / Voluntary
Students, Researchers, Regulators and policy-makers, Teachers and educators, Technicians, Managers, Scientific officers / Project managers, Professionals (e.g. veterinarians), General public
Academia, Industry, Governmental bodies, Contract Research Organizations (CROs), Consulting, SMEs
University (Bachelor), University (Master), University (Doctoral education), Postdoctoral (teaching and research), Continuing Professional Development
In vitro methods
Full coverage (a dedicated course)
|Details on the topic or technology covered:||
PBMCs play a critical role in modern biomedical research; however, the functional activity of these cells is highly variable
Many factors affecting PBMC variability, including genetic diversity and environmental pressure, are outside of researchers’ control
Conducting pre-screening and establishing PBMC functional activity profiles offers an attractive solution to address PBMC variability
Did you find what you were looking for?Yes, I found it! No, I did not!
Thanks for your feedback! Please note that we cannot respond unless you supply your email address.
What are you looking for?
Please give us your feedback so we can improve the information on the page. Thank you in advance for your help. Please add your email address if you would like a reply.Please contact us by email if you have any questions.