Microinjection into adherent cells - theory and practical exercises
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a dataset collected by the EU Commission in June-September 2018
. Some of these links will therefore die out with time.
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Owner/Developer: Eppendorf and European Molecular Biology Laboratory
| Country: |
India |
|---|---|
| Languages: |
English |
| Url: |
https://www.eppendorf.com/IN-en/service-support/eppendorf-training-center/details/training/show/419-microinjection-into-adherent-cells-theory-and-practical-exercises/ |
| Created: |
14 November 2018 |
| Locations: |
Germany |
| City: |
Heidelberg |
| Format: |
Hands-on training, Lecture |
| Presence: |
Optional / Voluntary |
| Access: |
Fee-based |
| Content type: |
Theoretical, Practical |
| Duration: |
2 days |
| Group size: |
8 |
| Frequency: |
Recurrent event |
| Prerequisites: |
Technicians and scientists interested in microinjection into adherent cells. Basic experience with cell culture and microscopy are required. |
| Target audience: |
Students, Researchers, Regulators and policy-makers, Teachers and educators, Technicians, Managers, Scientific officers / Project managers, Professionals (e.g. veterinarians) |
| Target sectors: |
Academia, Industry, Governmental bodies, Contract Research Organizations (CROs), Consulting, SMEs |
| Educational level: |
Continuing Professional Development, University (Doctoral education), Postdoctoral (teaching and research) |
| 3rs relevance: |
Replacement |
| Topics covered: |
In vitro methods |
| 3rs coverage: |
Full coverage (a dedicated course) |
| Course level on animal species: |
Advanced course |
| Details on the topic or technology covered: |
Content of seminar: A - Microinjection techniques into adherent cells: Sample preparation Microinjection of marker dyes Microinjection of GFP constructs B - Theoretical and practical microscopy training This part of the course will be supported by Carl Zeiss Microscopy C - Analysis of the experiments using fluorescence microscopy techniques |
| Learning outcome: |
By the end of the course, participants should be prepared to perform and analyze microinjection experiments into cultured cells using specific manipulators. |
| Qualification received: |
certificate of attendance |
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