Endocrinology

Source

Non-harmful / below threshold / severity degree 0 Mild / severity degree 1 Moderate / severity degree 2 Severe / severity degree 3
Federal Food Safety and Veterinary Office FSVO (2018)

  Administration of the test substance with subsequent blood sampling once or at intervals and frequencies imposing only mild short-term constraint, or euthanasia for the determination of hormone concentrations in the blood.

Models giving rise to clinically manifest endocrine disturbances in the animal, with suitable treatment.

Examples: Hypophysectomy. Adrenalectomy. Thyroidectomy. Parathyroidectomy. Spontaneous diabetes mellitus with clinical findings. Obese mouse with diabetes mellitus.
Lesions of the vagus nerve. Streptozocin.

Models giving rise to severe clinically manifest endocrine disturbances in the animal (decompensation) without suitable treatment (hormone substitution).

Examples: Hypophysectomy. Adrenalectomy. Thyroidectomy. Parathyroidectomy. Alloxan diabetes. Hyperthyroidism.
Hypercorticism.
Working Group of Berlin Animal Welfare Officers (2010)    

Diabetes with treatment.

Diabetes without treatment.

 

Bone metabolism

Source

Non-harmful / below threshold / severity degree 0 Mild / severity degree 1 Moderate / severity degree 2 Severe / severity degree 3
Federal Food Safety and Veterinary Office FSVO (2018)

Examples: Administration of vital stains with known innocuous properties in the drinking water or food for the study of dental or osseous development ex vivo. Examples: Determination of ossification or bone resorption ex vivo in the rat, after repeated administration of the test substance p.o. Determination of ossification in the mouse by marking of matrix synthesis, after repeated substance administration p.o. Examples: Ovariectomised rats for induction of bone-matrix loss. Long-term models giving rise to severe skeletal deformations or fractures in the animal.

 

Glucose metoabolism

Source

Non-harmful / below threshold / severity degree 0 Mild / severity degree 1 Moderate / severity degree 2 Severe / severity degree 3
Federal Food Safety and Veterinary Office FSVO (2018)

Fasting (water ad libitum) of adult animals of at least normal weight for max. 3 hours followed by a small blood sample.

Examples: Fasting Plasma Glucose (FPG) test

Test methods with applications and blood samples at intervals and frequencies imposing mild short-term constraint on the animals, under slightly prolonged reduced housing conditions.

Examples: Measurement with injection or gavage of a metabolic substrate or hormone followed by successive blood sampling over a few hours. Oral or intraperitoneal glucose tolerance test (OGTT, IGTT). Intraperitoneal insulin sensitivity test (IPIST).

Test methods with applications and blood samples at intervals and frequencies imposing moderate short-term constraint on the animals, under slightly prolonged reduced housing conditions.

Examples: Euglycemic clamps. Hypoglycemic clamps with subsequent euthanasia.
 

 

This page was updated on 01 January 2021

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