Cell Culture Trouble Shooting Course
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|Description:||This course provides you with knowledge and techniques to identify and avoid cell culture problems such as slow cell growth, reduced survival after thawing, and contaminations. You will be introduced to documentation and standardization methods as tools to optimize cell culturing. During the practical section, we will analyze the effects of the most common mistakes in routine cell culture, e.g., incorrect protocols and optimal freezing, thawing, and subcultivating cells, as well as mistakes resulting from the mishandling of cell culture media. Furthermore, you will be trained to early recognize contaminations and to avoid them by adopting certain standard procedures.|
Hands-on training, Lecture
Optional / Voluntary
cell culture experience
Students, Researchers, Regulators and policy-makers, Teachers and educators, Technicians, Scientific officers / Project managers
Academia, Industry, Governmental bodies, Contract Research Organizations (CROs), Consulting, SMEs
Continuing Professional Development, University (Master), University (Doctoral education), Postdoctoral (teaching and research)
In vitro methods
Full coverage (a dedicated course)
|Details on the topic or technology covered:||
The theoretical part includes:
Basics of sterile working techniques
Routine methods in cell culture
Components of media and their use
Cell counting and viability testing
Contaminations in cell cultures
Development of a trouble shooting guide
The practical part contains:
Thawing of cells with and without errors
Subcultivation of cells with and without errors
Freezing of cells with and without errors
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