Mouse embryos: primary phenotyping
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Owner/Developer: Mouse Clinical Institute
|Description:||This training is aimed at providing a theoretical and practical background knowledge destined for researchers and engineers that are willing to acquire primary expertise in mouse development. The topics will cover dissection of post implant embryos, histology, whole mount Lac Z staining and confocal imaging of whole embryos.|
Hands-on training, Lecture
Optional / Voluntary
Researchers, engineers, scientists. Need to have the basic knowledge in animal biology to understand mouse development physiology and the biological technics for phenotyping.
Students, Researchers, Teachers and educators, Technicians
Academia, Industry, Governmental bodies, Consulting, SMEs
In vitro methods
Partial coverage (e.g. a module)
|Details on the topic or technology covered:||
Phenotyping of mouse embryos
Determining the window of lethality of mutant mice in utero
Introduction to Mouse embryonic development
Classical histology (Fixation, embedding, orientation, sectioning and staining),
Immunohistochemistry and in situ hybridization
Imaging technics (HREM, micro CT, OPT)
High frequency echography to visualize and quantify embryonic development
The in-vitro culture of mouse embryos/ drug testing
Confocal macroscopy: application in embryology
Collection and fixation of mouse embryos and placentas at E10.5 (mid gestation stage)
Whole mount Lac Z staining of E10.5 embryos (Step1)
Observation of embryos by echography
Observation of embryos by micro CT, OPT (potentialy)
Whole mount Lac Z staining of E10.5 embryos (step 2) + observation
Collection of mouse embryos at E8.5 (early embryonic stage)
Collection of mouse fetuses and placentas at E18.5 (just before birth)
Observation of embryos at the confocal macroscope
Visualization of histological sections at different stages of development. Example of abnormalities.
Acquire primary expertise in mouse development.
Be able to design primary phenotyping experiment on mouse embryos.
Learn about all the primary phenotyping analysis on mouse embryos.
Dissect of post implant embryos.
Determine the window of lethality in utero.
Value embryos viability at neonatal step.
Observe embryos by echography.
Image embryos by MicroCT.
This training course is an « action d’adaptation et de développement des compétences » (ref. French law). A certificate of attendance is delivered after the course. An evaluation measures the trainees’ satisfaction and the achievement of learning objectives.
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