Films and slide shows of procedures on laboratory animals

This page contains links to older films and slide shows produced at the Norwegian School of Veterinary Science, and links to newer resources produced by international colleagues. They illustrate basic procedures (handling, injection techniques and blood sampling) on common laboratory animal species. Please note that refinements of these techniques are continuously being developed (see under other resources on this page).

Some of the animals were sedated before they were filmed. This was done purely to make it easier to demonstrate the various stages of each technique.

Please note: quality assurance of handling and procedures is just one part of the process of planning animal studies. For more information and resources on planning animal research and testing, please see the PREPARE guidelines. In particular, the section on Experimental Procedures contains links to additional resources for refinement of the techniques mentioned below.

Hen:
Handling and blood sampling; s.c. injection and cardiac puncture

Mink:
i.v. injection
Blood sampling from the saphenous vein

Mouse:
Immobilisation and blood sampling from the saphenous vein; i.p. injection; s.c. injection and oral gavage
Links to other resources:
Refinement of the method of handling mice for blood sampling from the saphenous vein
Refinement of the scruffing technique
Housing, handling and training mice at RISE (Research Institutes of Sweden) - includes refinement of oral gavaging, saphenous venepuncture of mice and blood sampling from the tail of mice and rats (see also their film Refinement work at RISE)
Micropipette-guided drug administration (MDA) as an alternative to gavaging
Mouse handling tutorial (Kelly Gouveia, John Waters & Jane Hurst, University of Liverpool)
Mouse handling video clips from the NC3Rs
Webinar: Mouse handling made easy - Reducing anxiety in mice and their handlers
Advice on rodent housing from NA3RsC
Oral gavage training (Instech)
Automated blood sampling (Instech)
Portal vein catheterisation in mice

Pig:
Immobilisation and blood sampling
The Panepinto Sling for minipigs and other animals up to 150 kg

Links to other resources:
Handling, Dosing and Training of the Göttingen Minipig
Articles and posters from the Ellegaard Göttingen Minipigs Knowledge Base
Panepinto (2020): Prioritizing humane minimum-stress methods in swine facility and protocol design

Rabbit:
Handling, lifting and immobilisation; blood sampling fron the central ear artery; i.v. injection; s.c. injection and terminal bleeding (cardiac puncture)
Links to other resources:

Advice on rabbit handling and housing from NA3RsC

Rat:
Handling, lifting and immobilisation; how to wrap up a rat; s.c. and i.p. injections; blood sampling from the saphenous vein
Links to other resources:
Refinement of the scruffing technique
Refinement of the method of handling mice for blood sampling from the saphenous vein (relevant for rats as well)
Anatomy of the rat and Anatomía de la rata produced by the University of Alcalá, Madrid
Investigation of a Mammal (Rat): a dissection film
Rat tickling (Course with certificate) and more background information
Housing, handling and training rats at RISE (Research Institutes of Sweden) (see also their film Refinement work at RISE)
Using Clicker Training and Social Observation to Teach Rats to Voluntarily Change Cages
Procedures with Care
Oral gavage training (Instech)
Advice on rodent housing from NA3RsC

Intubation of the hamster

Guidance on necropsies of laboratory animals in the PREPARE guidelines

General resources

More guidance on blood sampling

The Blood Sampling site produced by the National Centre for the 3Rs (NC3Rs), UK contains many resources and literature references.

Novo Nordisk have produced videos showing methods of housing mice, rats, guinea pigs, rabbits, dogs (indoors and outdoors) and pigs (indoors and outdoors).

Guidelines for a range of procedures can be found in the 3R Guide database by using the search engine.
This page was updated on 08 October 2021

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