Source

Directive 2010/63/EU, Annex VIII

Federal Food Safety and Veterinary Office FSVO (2018)

Sampling of blood, saliva or urine (non-invasive) without sedation, at intervals and frequencies or in volumes imposing no constraint on the animals (no prolonged restraining measures, no other interventions or previous administrations of test substances). Collection of body fluids, tissues, organs, or body parts under deep general anaesthesia directly followed by euthanasia in animals not previously subjected to any intervention.

Examples: Collection of blood samples from the ear vein of the rabbit, twice with an interval of 14 days, 3 ml on each occasion. Single non-invasive swabs of body cavities.

Lege artis collection of blood, tissue, faeces or urine, with or without sedation, at intervals and frequencies imposing mild short-term constraint on the animals with non-toxic doses of test substances, slightly prolonged reduced housing conditions. Examples: Several blood samples from the tail vein, saphenous vein or sublingual vein in the mouse and rat within 24 hours. Skin biopsies. Perfusion of small rodents under terminal surgical anaesthesia.

Sampling of blood in volumes and at intervals and frequencies causing moderate short-term constraint on the animals; percutaneous urine collection, collection of tissue samples (cells or fluid), peritoneal washing of naïve animals under general anaesthesia. Sampling of body fluids (in relatively large quantities, in relatively large numbers or at relatively short intervals) after administration of pharmacologically active substances (no toxic doses, no other interventions, no prolonged restraining measures).

Examples: Repeated daily collection of blood samples from the tail vein in rats over five days to determine the course of hormone levels.

Federal Food Safety and Veterinary Office FSVO (2018)

Collection of fluids or tissue resulting in mild impairment of the young animals’ further development. 

Examples: Venous blood collection in premature calves

Collection of fluids or tissue resulting in moderate impairment of the young animals’ further development.

Collection of fluids or tissue resulting in severe impairment of the animals’ further development or death/abortion.

Working Group of Berlin Animal Welfare Officers (2010)

In small quantities and small numbers with only short-term compulsory measures and not combined with other procedures: Venipuncture. Foley catheter for larger animals.

In small quantities and small numbers with only short-term compulsory measures and not combined with other procedures.

With severe restrictions on motion and not combined with other procedures.

Working Group of Berlin Animal Welfare Officers (2010)

Under anaesthesia only: Final puncture of the heart.

Under anaesthesia only: Venipuncture with indwelling catheter: bladder aspiration. Gallbladder aspiration. Bone marrow aspiration. Peritoneal lavage. Retrobulbar (repeated puncture of the same eye after a fortnight latency). Indwelling cannula in the cisterna magna.

Source

Directive 2010/63/EU, Annex VIII

Superficial procedures, e.g. ear and tail biopsies.

Home Office (2014 b)

Invasive genotyping carried out using a method that causes only transient pain, even if the phenotype is not harmful.

Federal Food Safety and Veterinary Office FSVO (2018)

Experimental marking and genotyping using non-invasive methods.

Examples: Marking with dye. Genotyping by hair sample.

Experimental marking using invasive methods and tissue collection under anaesthesia for the genotyping of adult animals.

Examples: Tattooing. Ear punching, Microchips in rodents and rabbits. experimental tail tip biopsy up to 0.5 cm. Toe tip amputation up to the age of 3 weeks.

Experimental interventions to regulate reproduction or for genotyping of adult animals.

Examples: Vasectomy. Castration.